HPLC (High-Performance Liquid Chromatography)

HPLC (High-Performance Liquid Chromatography) is the gold standard analytical technique for measuring peptide purity. It works by passing a dissolved sample through a column packed with stationary-phase particles; different compounds travel at different speeds based on their chemical interactions with the stationary phase and are detected by UV absorbance (typically at 214 nm for peptide bonds) as they elute. The resulting chromatogram shows distinct peaks; purity is calculated as the target compound''s peak area as a percentage of total peak area. For research-grade peptides, ≥98% HPLC purity is the accepted minimum threshold, meaning ≤2% of the detected material is impurities, degradation products, or residual synthesis byproducts.

Related peptides: All Aevitas peptides are ≥98% HPLC verified.

Part of the Aevitas Peptide Glossary.